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. 2011 Oct 5;86(1):14. doi: 10.1095/biolreprod.111.094037

FIG. 3.

FIG. 3.

SLC26A3, SLC26A6, and SLC9A3R1 interact with CFTR in mouse sperm. Purified sperm membranes were used to immunoprecipitate SLC26A3, SLC26A6, CFTR, and SLC9A3R1. The input (SM), the supernatant (S), and the eluted fraction (E) were loaded in 7% acrylamide gels, blotted, and probed by Western blot assays with anti-SLC26A3 (A), anti-SLC26A6 (B), anti-SLC9A3R1 (C), and anti-CFTR (D). Molecular weight markers (in kDa) are shown on both sides of each panel. An immunoprecipitation negative control was performed in each case using PDHE1β (shown at the right of each panel). Expected sizes: 98, 95, 55, 165, and 37 kDa for SLC26A3, SLC26A6, SLC9A3R1, CFTR, and PDHE1β, respectively. The 50-kDa band in blots for SLC26A3, SLC26A6, and CFTR and the 25-kDa band in the blot for SLC9A3R1 correspond to the light chains from IgGs, which were detected by the secondary antibody used in each case (goat and rabbit, respectively). These blots are representative of three independent experiments.