Effects of simvastatin (1 μM), an inhibitor of farnesylation (FTI; 10 μM), an inhibitor of geranylgeranylation (GGTI; 10 μM), and 22-hydroxycholesterol (10 μM) on the mRNA expression of Star (A), Cyp11a1 (B), Hsd3b1 (C), and Cyp17a1 (D). The cells were cultured for 48 h in chemically defined media supplemented with LH (5 ng/ml) in the absence (control) or in the presence of simvastatin, GGTI, FTI, and/or 22-hydroxycholesterol. The mRNA expression was determined as described in Figure 1. Results are presented as a percentage of control. Each bar represents the mean ± SEM. Means with no superscripts in common are significantly different (P < 0.05).