Skip to main content
. 2011 Sep 14;86(1):22. doi: 10.1095/biolreprod.111.093864

FIG. 4.

FIG. 4.

In vivo analysis of male fertility and sperm fertilizing capacity is shown. A) A limited mating trial was performed to determine whether Fyn-null males were fertile. Adult Fyn-null or WT males (n = 4) were each caged with two CF1 females, as described in Materials and Methods. The mean ± SD number of fetuses per female is indicated by vertical bars. Analysis by t-test indicated that differences between means were not significant (P > 0.05). B) The relative fertilizing capacities of sperm from three WT and Fyn-null males were tested by artificial insemination of CF1 females by using an equal mixture of Fyn-null and WT sperm. Genotype analysis of fetuses resulting from artificial insemination detected the presence of the neomycin insert in those fetuses produced from Fyn-null sperm (center). The percentage of fetuses per female derived from Fyn-null sperm is presented as dark grey in the pie chart (right). The percentage of fetuses derived from Fyn-null sperm was significantly lower than that derived from WT sperm, as determined by t-test (P < 0.001).