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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Biomaterials. 2011 Oct 1;32(36):9612–9621. doi: 10.1016/j.biomaterials.2011.09.012

Figure 3. Differentiation of hESCs on scaffolds of varying stiffness, as determined using the Applied Biosystems hESC array.

Figure 3

Figure 3

Figure 3

Figure 3

After two weeks in culture, total RNA was extracted from homogenized hESC-seeded scaffolds. Expression of selected genes representative of the mesoderm (a), endoderm (b) and ectoderm (c) germ layers was measured and are presented as described in Figure 2. Ratios are presented as mean values (±SD) of gene expression levels determined from duplicates. (d–f)Immunohystochemistry of selected mature markers expression on: (d) LoEM, (e) IntEM and (f) HiEM scaffolds.