Figure 2.
Synapses in developing biglycan null mice are similar to wild-type. A, Postsynaptic specializations in fetal biglycan null mice. Diaphragms were dissected from E16 biglycan null mice and labeled with rh-α-bungarotoxin to visualize the AChRs. Discrete AChR clusters are arrayed in a central band across the muscle. This morphology and localization are indistinguishable from that observed in wild-type muscle at this age (data not shown). Scale bar, 20 μm. B, Diaphragms were dissected from P0 and P14 heterozygous (top) and biglycan null (bottom) littermates and double labeled with rh-α-bungarotoxin to visualize the AChRs (red) and anti-synaptophysin/anti-neurofilament (green) to show the nerve. In each set the merged and AChR-only images are shown at left and right, respectively. No structural differences were observed in the synapses from these developing biglycan null mice compared with their normal littermates. Scale bar, 10 μm.