Figure 6.

Photomicrographs of macaque brain sections stained with plasma that produced one of several infrequently observed patterns of immunoreactivity (Part I). (A) A section through the dentate gyrus processed with plasma from an individual with ASD showing specific staining of hilar neurons of the polymorphic layer. (B) Section processed with plasma that produced staining of axons with a beaded morphology in the polymorphic layer of the dentate gyrus (arrows). This section was processed with plasma from a TD subject. (C) A section stained with plasma from a TD subject in which axons are selectively immunolabeled, with several labeled axons in the somatosensory cortex shown here (arrows). (D) A different plasma sample, from a TD subject, produced blood vessel labeling throughout the brain. Labeling (arrows) in the cerebellum is shown here. (E) A section through the cerebellum processed with plasma from a TD subject showing intense immunoreactivity to the cell bodies (*) and dendrites (arrows) of the Purkinje cells. (F) Immunolabeling of the basket plexus (arrows) surrounding Purkinje cells in the cerebellum in the molecular layer. Cell bodies of the Purkinje cells are surrounded by the basket plexus and are denoted by a ‘*’. This tissue was processed with plasma from a subject with ASD that resulted in labeling of neurofilaments throughout the brain. Each calibration bar = 100 m. gl = granule cell layer of dentate gyrus, gcl= granule cell layer of cerebellum, ml = molecular layer of dentate gyrus, mol= molecular layer of cerebellum, pl = polymorphic layer of dentate gyrus. I, II, and III refer to the first, second, and third layers of neocortex.