Table 2.
Lipid composition of various membranes from T. pyriformis NT-1 cells grown at different temperatures
Cilia | Pellicles | Microsomes | |||||||
---|---|---|---|---|---|---|---|---|---|
15 ℃ | 24 ℃ | 39 ℃ | 15 ℃ | 24 ℃ | 39 ℃ | 15 ℃ | 24 ℃ | 39 ℃ | |
Phospholipid composition | |||||||||
2-Aminoethylphosphonolipids (AEPL) | 40.0 | 37.0 | 36.4 | 30.8 | 32.9 | 19.1 | 22.9 | 20.8 | 14.7 |
Phosphatidylethanolamines (PE) | 20.8 | 21.4 | 20.0 | 33.2 | 36.2 | 49.2 | 34.1 | 35.5 | 43.9 |
Phosphatidylcholines (PC) | 15.8 | 18.1 | 30.0 | 18.9 | 21.4 | 23.5 | 30.0 | 27.6 | 32.4 |
Fatty acid composition of phospholipids | |||||||||
C14:0 | 6.0 | 3.9 | 6.7 | 8.4 | 9.6 | 8.9 | 7.2 | 7.4 | 7.3 |
C16:0 | 16.8 | 19.4 | 16.8 | 12.7 | 15.8 | 16.4 | 8.9 | 10.4 | 13.2 |
C16:1Δ9 | 14.4 | 7.7 | 8.3 | 9.9 | 7.4 | 7.1 | 8.8 | 9.8 | 8.7 |
C18:0 | 2.5 | 3.8 | 6.1 | 0.7 | 2.0 | 3.0 | 0.5 | 1.4 | 2.2 |
C18:1Δ9 | 6.9 | 8.0 | 11.7 | 7.4 | 8.5 | 12.6 | 8.1 | 7.1 | 13.9 |
C18:2Δ9,12 | 9.0 | 9.7 | 8.1 | 16.2 | 13.8 | 10.5 | 19.5 | 17.7 | 13.0 |
C18:3Δ6,9,12 | 27.2 | 28.2 | 20.7 | 28.0 | 27.4 | 18.5 | 31.8 | 31.2 | 21.4 |
Data from Fukushima et al. (1976).13)