Table 4.
Palmitoyl-CoA and stearoyl-CoA desaturase activities in T. pyriformis NT-1 microsomes from control and cycloheximide-treated cells after temperature shift-down
Growth condition | nmol/min/mg protein | |||
---|---|---|---|---|
Palmitoyl-CoA desaturase | Stearoyl-CoA desaturase | |||
Control | Cycloheximide | Control | Cycloheximide | |
39 ℃-cells | 0.90 | 0.65 | 6.31 | 4.80 |
After shift (39 ℃ → 15 ℃) | ||||
0 h | 2.29 | 0.74 | 12.25 | 4.65 |
1 h | 3.40 | 0.39 | 15.50 | 6.20 |
2 h | 3.90 | 0.24 | 17.84 | 4.10 |
4 h | 2.07 | 0.15 | 4.90 | 2.65 |
6 h | 0.54 | 0.08 | 2.70 | 1.30 |
10 h | 0.32 | 0.05 | 1.50 | 0.70 |
24 h | 0.77 | — | 5.85 | — |
15 ℃-cells | 0.59 | 5.00 |
Data from Umeki et al. (1982).28)