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. 2011 Oct 11;87(8):563–573. doi: 10.2183/pjab.87.563

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© 2011 The Japan Academy

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Depletion of NEDD4 decreased the expression of collagen and fibronectin. (A) Type 1 or 3 collagen and fibronectin expression was diminished in NEDD4 knocked down NHDF cells by RT-PCR. (B) The extracellular fibronectin was detected by immunoblot analysis using cell conditioned culture media. The protein level of fibronectin also decreased in NEDD4 depleted NHDF cells both extra- and intracellular compartment. CM; conditioned media. (C) The collagen expression was analyzed 72h after transfection of expression vector. The protein level of collagen was increased in NEDD4 over-expressing NIH3T3 cells than mock cells.