TABLE 1.

iTRAQ Labeling Scheme for the Normalization Experiment

iTRAQ label	Protein quantity to be used in pooling (μg)	Approximate volume (μL) of 100 μg digested proteins after Speed Vac	Ethanol to add to labeling reaction (μL)	Approximate volume of iTRAQ labeling reagent (μL)	Water to add to quench labeling reaction (μL)	Volume of label and digest after quenching (μL)	Volume to match protein quantity desired in pooling (μL)
114	5	40	70	35	100	246	12.3
115	25	40	70	35	100	250	62.5
116	50	40	70	35	100	245	122.5
117	100	40	70	35	100	Combined all labels into this vial	Combined all labels into this vial