TABLE 5.
SwissProt entry name | Protein mass | 4 114 | 4 115 | 4 116 | 4 117 | 3 114 | 3 115 | 3 116 | 3 117 | CV % |
---|---|---|---|---|---|---|---|---|---|---|
EFTU1_ECO24 | 46886 | 1.30 | 1.31 | 1.34 | 1.32 | 1.31 | 1.40 | 1.37 | 1.30 | 2.6 |
TNAA_ECOBW | 57462 | 1.53 | 1.41 | 1.45 | 1.51 | 1.43 | 1.44 | 1.47 | 1.49 | 2.6 |
ODP1_ECO57 | 107009 | 1.28 | 1.36 | 1.53 | 1.22 | 1.38 | 1.32 | 1.21 | 1.35 | 7.5 |
ATPB_ECOBW | 53377 | 0.90 | 0.86 | 0.89 | 0.91 | 1.03 | 0.89 | 0.92 | 0.89 | 5.7 |
RPOC_ECODH | 168599 | 0.80 | 0.88 | 0.98 | 0.78 | 0.78 | 0.82 | 0.79 | 0.87 | 8.3 |
EFG_ECOBW | 84188 | 0.90 | 0.83 | 0.83 | 0.84 | 0.82 | 0.81 | 0.89 | 0.83 | 3.9 |
RS1_ECO57 | 67575 | 1.06 | 1.08 | 1.12 | 1.13 | 1.10 | 1.12 | 1.14 | 1.10 | 2.4 |
TIG_ECOBW | 53351 | 1.22 | 1.15 | 1.16 | 1.25 | 1.17 | 1.20 | 1.24 | 1.20 | 2.9 |
PFLB_ECOLI | 92937 | 1.36 | 1.35 | 1.45 | 1.40 | 1.23 | 1.27 | 1.33 | 1.44 | 5.6 |
OMPA_ECOLI | 40174 | 0.92 | 1.37 | 1.03 | 0.94 | 0.88 | 1.01 | 0.98 | 1.07 | 14.7 |
ENO_ECOBW | 51159 | 0.83 | 0.81 | 0.74 | 0.92 | 0.93 | 0.87 | 0.92 | 0.83 | 7.7 |
TALB_ECOLI | 38683 | 0.99 | 1.00 | 1.07 | 0.90 | 1.10 | 0.91 | 0.92 | 0.99 | 7.5 |
CH60_ECO24 | 63372 | 1.07 | 0.93 | 0.92 | 1.02 | 0.93 | 0.94 | 0.95 | 1.00 | 5.6 |
RBSB_ECOLI | 34822 | 0.73 | 0.70 | 0.73 | 0.73 | 0.71 | 0.66 | 0.71 | 0.74 | 3.6 |
RPOB_ECOBW | 162610 | 0.90 | 0.84 | 0.75 | 0.90 | 0.85 | 0.87 | 0.93 | 0.83 | 6.5 |
RL7_ECO24 | 14305 | 0.44 | 0.38 | 0.37 | 0.44 | 0.39 | 0.40 | 0.48 | 0.40 | 9.3 |
HTPG_ECOHS | 78151 | 0.86 | 0.84 | 0.74 | 0.81 | 0.83 | 0.81 | 0.78 | 0.81 | 4.5 |
CLPB_ECOLI | 102182 | 0.49 | 0.54 | 0.54 | 0.47 | 0.48 | 0.51 | 0.49 | 0.50 | 5.3 |
GRCA_ECOBW | 15485 | 1.34 | 1.41 | 1.51 | 1.34 | 1.32 | 1.36 | 1.38 | 1.40 | 4.4 |
ODP2_ECOLI | 73893 | 0.80 | 0.74 | 0.74 | 0.76 | 0.67 | 0.76 | 0.75 | 0.76 | 4.9 |
PUR9_ECO24 | 61944 | 0.47 | 0.42 | 0.37 | 0.45 | 0.42 | 0.45 | 0.48 | 0.41 | 8.4 |
6PGD_ECOLI | 55742 | 0.58 | 0.60 | 0.50 | 0.57 | 0.60 | 0.59 | 0.56 | 0.56 | 5.8 |
KPYK1_ECO57 | 56083 | 0.70 | 0.72 | 0.72 | 0.66 | 0.69 | 0.67 | 0.67 | 0.69 | 2.8 |
SYGB_ECOBW | 82988 | 0.55 | 0.59 | 0.64 | 0.56 | 0.60 | 0.55 | 0.57 | 0.59 | 5.7 |
SYP_ECOBW | 67889 | 0.33 | 0.36 | 0.37 | 0.33 | 0.23 | 0.31 | 0.34 | 0.36 | 13.6 |
ATPA_ECOBW | 59018 | 0.54 | 0.49 | 0.43 | 0.52 | 0.52 | 0.50 | 0.53 | 0.49 | 6.8 |
GLYA_ECOBW | 49782 | 0.64 | 0.57 | 0.55 | 0.62 | 0.57 | 0.57 | 0.62 | 0.60 | 5.5 |
SYD_ECOBW | 70912 | 0.57 | 0.57 | 0.52 | 0.64 | 0.54 | 0.65 | 0.59 | 0.58 | 7.8 |
RL9_ECO24 | 17489 | 0.50 | 0.45 | 0.43 | 0.47 | 0.54 | 0.47 | 0.44 | 0.46 | 7.2 |
PURA_ECOBW | 50858 | 0.60 | 0.67 | 0.71 | 0.60 | 0.61 | 0.65 | 0.63 | 0.64 | 6.1 |
Average CV % | 16.1 |
Each column has been normalized to the sum of 100. Proteins (471) were found in common between the two experiments. Only the top 30 abundant proteins are shown in this table to illustrate the feasibility of combining individual, deconvoluted protein quantitation of each sample to enable a new, proteomic analysis without the iTRAQ-labeled samples having been run in the same experiment.