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. 2001 Apr 10;98(9):4910–4915. doi: 10.1073/pnas.081288198

Figure 3.

Figure 3

FHV RNA accumulation in inoculated and noninoculated leaves of MP-transgenic N. benthamiana plants. FHV RNA2 (1 μg) purified from virions (positive control) or total RNAs (1–5 μg) extracted from leaves were used as templates for RT-PCR with FHV RNA2 specific primers, as described in the text. Products were analyzed by electrophoresis on 1% agarose gel. Lanes: M, 1-kb ladder; C, buffer-inoculated leaf (negative control); F, FHV RNA2; 1, nontransgenic plant, FHV RNA inoculated; 2–4, TMV MP transgenic plant, FHV RNA inoculated (2) and noninoculated upper second (3) and third (4) leaves; 5–7, RCNMV MP transgenic plant, FHV RNA inoculated (5) and noninoculated upper second (6) and third (7) leaves. The arrow shows the position of FHV RNA2 (1.4 kb).