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. 2012 Jan 3;21(8):1770–1781. doi: 10.1093/hmg/ddr610

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© The Author 2012. Published by Oxford University Press

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Incomplete ubiquitination is not due to impaired parkin translocation to mitochondria. (A) Anti-ubiquitin immunoblots of liver mitochondrial extracts obtained from MSD (n = 4) and control (n = 4) mice at 1 month and 3 months. ATPase β was used as mitochondrial loading control and results were expressed as the fold changes of the ubiquitin/ATPase β ratio; *P < 0.05. (B) Confocal images (63×) of untreated (NT) and CCCP-treated (20 μm, 20 h) MSD and control MEFs co-expressing GFP-parkin (green) and DsREDmito (red) plasmids. The zoomed region is indicated with a white square. Co-localization is expressed as the M1 (Mander's) index. ANOVA P-value = 0.158, **P < 0.01.