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. 2012 Jan 3;21(8):1770–1781. doi: 10.1093/hmg/ddr610

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© The Author 2012. Published by Oxford University Press

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Reduced parkin levels are not a consequence of low Park2 expression. (A) Parkin total levels in liver homogenates from MSD (n= 4) and control (ctrl) mice (n= 4) at 1 month and 3 months. Relative levels are expressed as the Parkin/actin ratio in terms of fold changes; **P < 0.01. (B) Parkin distribution in subcellular fractions (c, cytosol; m, mitochondria) obtained from liver of MSD (n= 4) and control (ctrl) mice (n= 4) at 3 months. Mitochondrial levels were normalized with COX IV (mitochondrial loading control) and the Parkin/COX IV ratio was expressed as fold changes; *P < 005. (C) Park2 mRNA relative levels in MSD liver (n= 3) at 1 month and 3 months obtained by quantitative PCR. Values are expressed as fold changes compared with control; ANOVA P-value = 0.2.