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. 2001 Apr 10;98(9):4916–4921. doi: 10.1073/pnas.081072798

Figure 2.

Figure 2

Plasmids used for in vitro translation inhibition assays. (a) The bicistronic constructs under the control of a T7 transcriptional promoter contained the Renilla luciferase (R-Luc) reporter gene, a viral IRES element, and the chloramphenicol acetyltransferase (CAT) reporter gene. The viral sequences incorporated in these constructs consisted of the 5′ UTRs of EMCV (586 nucleotides), CSFV (373 nucleotides), and HCV (341 nucleotides), respectively. (b) The monocistronic construct (T368-CAT) contained the HCV IRES sequence, consisting of the entire 5′ UTR (341 nucleotides) and 27 nucleotides of the polyprotein coding sequence, and the CAT reporter gene cloned into the pGEMT-Easy (Promega) expression vector.