Skip to main content
. 2012 Mar 14;32(11):3697–3711. doi: 10.1523/JNEUROSCI.5640-11.2012

Figure 7.

Figure 7.

BLOC-1 deficiences modulate peroxiredoxin levels and cell redox state. A–E, Dentate gyri of wild-type (Dtnbp1+/+) and BLOC-1 deficient (Dtnbp1Sdy/Sdy) mice were analyzed for alteration in content or distribution of redox enzymes peroxiredoxin 1 and peroxiredoxin 2 (Prdx1, Prdx2). Fluorescence intensity is significantly reduced in the dentate region of BLOC-1 deficient mice. VAMP2 was used as positive control. E, Box plot depicts the fluorescent ratios in wild-type (Dtnbp1+/+) and BLOC-1 deficient (Dtnbp1sdy/sdy) mice. F, Reduction in Prdx1 and Prdx2 levels is recapitulated in SH-SY5Y cells where BLOC-1 is downregulated by shRNA. G, H, Flow cytometry quantification of cellular hydrogen peroxide in control shRNA and pallidin shRNA-treated cells using a fluorescent probe against hydrogen peroxide (DCF). As controls, cells were either not stained with the probe or treated with 2 μm hydrogen peroxide and stained with DCF. I, Ratios of Experimental and Control means of cell population fluorescence profiles depicted in G, H. Each dot represents an independent experiment performed with three independent shRNA knock-downs. One-way ANOVA, Dunnett's multiple comparison.