Figure 6.
KAI1 inhibits HIF-1α and CDCP1 expression and VEGF secretion in tumor xenografts. (A) PC3 cells stably transfected with KAI1 (PC3-KAI1 #5 andPC3-KAI1 #6) or vector control (PC3-GFP #8) were resuspended in PBS (2 × 106 cells/100 μl) and injected subcutaneously into the flanks of athymic nude mice. Animals were sacrificed 35 days after injection, at which time tumors were collected and photographed. Tumor volumes were measured using precision calipers (B) Photomicrographs showing expression of HIF-1α, CDCP1, and VHL protein in tumor sections by immunohistochemistry.