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Unnatural T7 transcription employing x⋅y pairing. (a) Schemes of the experiments. (b and c) Gel electrophoresis of transcripts with the templates (N = x or A) in the presence or absence of the natural and unnatural nucleoside triphosphates. (d–f) Two-dimensional TLC for nucleotide-composition analyses of transcripts isolated by the gel electrophoresis shown in b (lane 1) and c (lane 1). The quantitative data are shown in Table 1.
