Table 1.
Nucleotide-composition analysis of T7 transcripts
| The 11th base in template | rN′TP,
mM
|
Composition of nucleotides incorporated as 5′
neighbor of A*
|
|||||
|---|---|---|---|---|---|---|---|
| Natural (N′ = A, G, C, and U) | Unnatural | Gp | Ap | Cp | Up | Unnatural | |
| A | 1 | 1 (y) | 3.94 (4)† | 1.04 (1) | 1.00 (1) | 1.03 (1) | ND (0) |
| A | 1 | 2 (y) | 3.94 | 1.04 | 0.99 | 1.03 | ND |
| A | 2 | 2 (y) | 4.04 | 1.04 | 0.89 | 1.01 | ND |
| x | 1 | 1 (y) | 3.94 (4) | 1.07 (1) | 1.02 (1) | 0.05 (0) | 0.92 (1) |
| x | 1 | 2 (y) | 3.94 | 1.05 | 1.01 | 0.04 | 0.97 |
| x | 2 | 2 (y) | 3.91 | 1.05 | 1.01 | 0.04 | 0.99 |
| A | 1 | 1 (m5y) | 4.04 (4) | 1.05 (1) | 0.91 (1) | 0.99 (1) | ND (0) |
| A | 2 | 2 (m5y) | 4.01 | 1.03 | 0.93 | 1.03 | ND |
| x | 1 | 1 (m5y) | 4.01 (4) | 1.03 (1) | 0.97 (1) | 0.04 (0) | 0.95 (1) |
| x | 2 | 2 (m5y) | 3.98 | 1.07 | 0.98 | 0.03 | 0.93 |
*
The values were determined via the following formula: (radioactivity of each nucleotide)/(total radioactivity of all nucleotides) × [(total numbers of nucleotides at 5′ neighbor of A) = 7].
†
The theoretical number of each nucleotide is shown in parentheses.
ND, not detected (<0.01).