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. 2012 Mar 27;10(3):e1001287. doi: 10.1371/journal.pbio.1001287

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DuBois et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Heatmap for microarray following NUP-1 knockdown in BSF cells. Of 8,110 genes, 62 were increased in relative expression greater than 2-fold. Yellow and blue represent upregulation and downregulation, respectively, and black no expression change. Gene accession numbers/annotation are given for the upregulated genes. (B) qRT-PCR quantitation of the expression of EP and GPEET procyclin genes (left panel), selected ES VSG genes (middle panel), and housekeeping genes (right panel) during NUP-1 RNAi. The expression level in control cells was set to one, and y-axis is the relative expression compared to control cells, normalized to β-tubulin.