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. 2012 Mar 27;10(3):e1001287. doi: 10.1371/journal.pbio.1001287

Figure 9. Depletion of NUP-1 derepresses the entire expression site, but not all telomeric genes.

Figure 9

(A) Top: NUP-1 RNAi was induced in cells with a GFP:NPT reporter downstream of the repressed VSG 2 expression site promoter. Bottom: Expression of VSG 2 and the reporter increased as determined by qRT-PCR normalized to Rab11. (B) Top: NUP-1 RNAi was induced in a cell line with NPT reporter ∼2 kb upstream of a de novo telomere. Bottom: Expression site VSG gene expression increased but NPT expression did not as determined by qRT-PCR normalized to Rab11. Expression of VSG 2, the active VSG in this cell line, decreased.