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. 2012 Mar 27;6(3):e1583. doi: 10.1371/journal.pntd.0001583

Figure 1. MβCD treatment is effective in sequestering cholesterol from the plasma membrane.

Figure 1

(A) Control, (B) 15 mM MβCD treated and (C) 15 mM MβCD followed by 0.05 mM WSC treated cardiomyocytes show significant changes in Filipin labeling. Cholesterol-depleted cells (B) reveal very little Filipin labeling, whereas cholesterol-replenished cells show strong labeling for cholesterol, similar to control cells. (D) Cardiomyocytes treated with 5, 10 or 15 mM of MβCD reveal a substantial decrease in Filipin labeling in a dose-dependent manner whereas cholesterol replenishment with 0.05 mM of WSC returns Filipin fluorescence to control values. Normalized data are shown as mean of triplicates ±SD. Asteriks indicate statistically significant differences (* p < 0.05 and ** p < 0.01, One way ANOVA followed by Newman-Keuls) between control and treated cells. Scale bar: 10 µm.