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. 2012 Mar 27;6(3):e1595. doi: 10.1371/journal.pntd.0001595

Table 3. Yields of Ae.aegypti P450s expressed in E. coli.

P450 Na Expression Membrane Content
(nmol/L)b nmol P450/mgc nmol cyt c/min/mgd
CYP6CB1 3 10±7 0.1±0.1 37±24
CYP9J10 3 0 0 102
CYP9J19 1 186 1.56 32
CYP9J24 3 29±8 0.6±0.3 93±35
CYP9J26 2 25, 5 0.3, 0.1 45, 32
CYP9J28 3 47±30 1.1±0.9 157±65
CYP9J32 4 83±28 0.9±0.3 142±14
a

Number of independent membranes used for yield calculations. Measurements are expressed as means ± standard deviation. Where N<3, individual measurements are listed.

b

nmol of P450 isolated in membrane preparation per litre of bacterial cultures.

c

P450 concentration in membrane preparations as nmol of P450 per mg of total protein.

d

CPR activity in membrane preparation measured as nmol of cytochrome c reduced per min per mg of total protein.