Table 3. Yields of Ae.aegypti P450s expressed in E. coli.
P450 | Na | Expression | Membrane Content | |
(nmol/L)b | nmol P450/mgc | nmol cyt c/min/mgd | ||
CYP6CB1 | 3 | 10±7 | 0.1±0.1 | 37±24 |
CYP9J10 | 3 | 0 | 0 | 102 |
CYP9J19 | 1 | 186 | 1.56 | 32 |
CYP9J24 | 3 | 29±8 | 0.6±0.3 | 93±35 |
CYP9J26 | 2 | 25, 5 | 0.3, 0.1 | 45, 32 |
CYP9J28 | 3 | 47±30 | 1.1±0.9 | 157±65 |
CYP9J32 | 4 | 83±28 | 0.9±0.3 | 142±14 |
Number of independent membranes used for yield calculations. Measurements are expressed as means ± standard deviation. Where N<3, individual measurements are listed.
nmol of P450 isolated in membrane preparation per litre of bacterial cultures.
P450 concentration in membrane preparations as nmol of P450 per mg of total protein.
CPR activity in membrane preparation measured as nmol of cytochrome c reduced per min per mg of total protein.