Table 1. Affinity of CBM32-5 for carbohydrates determined at 20°C in 20 mM Tris HCl, pH 8.0.

Carbohydrate	Ka (M−1)
d-galactose	1.69 (±0.05)×103
d-GalNAc	5.01 (±0.64)×103
Lactose (Gal-β-1,4-Glc)	2.40 (±0.58)×103
Gal-β-1,3-GalNAc	3.77 (±0.34)×103
GalNAc-β-1,3-Gala	3.40 (±0.10)×103

^athe binding constant for GalNAc-β-1,3-Gal was determined by ITC; the remaining binding constants were determined by UV difference titrations.