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. 2012 Mar 27;7(3):e34043. doi: 10.1371/journal.pone.0034043

Figure 1. Gut microbiota comparison of inoculated mice.

Figure 1

Principal Component Analysis (PCA) plot based on DGGE profiles of 16S rRNA gene PCR derived amplicons of feces samples collected from mice in the inoculation experiment at nine weeks of age. Germ-free (GF) mice were inoculated at one week of age (red balls) or at three weeks of age (yellow balls) with a bacteria suspension made from caecal content of specific pathogen-free (SPF) mice from Taconic Farms (blue balls illustrate different aliquots of the suspension). Their gut microbiota was compared with SPF mice raised in our barrier (green balls). ANOVA based on Principal Component (PC) 1 explaining 14.7% of the variance confirmed a significant difference between mice inoculated at three weeks of age and all other mice (p<0.01). No significant differences of PC values were found between ex-GF mice inoculated at three weeks of age and the suspension aliquots used to inoculate these mice with. DGGE: Denaturing Gradient Gel Electrophoresis.