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. 2001 Apr 17;98(9):4926–4931. doi: 10.1073/pnas.091090798

Figure 1.

Figure 1

Characteristics of BCθ binding to intact cells. (a) BCθ binding depends on cholesterol contents in cell membranes. Cholesterol was depleted from platelets with 5, 10, and 20 mM 2OHpβCD. The amount of BCθ bound to the platelets was quantified by flow-cytometric analysis (Inset) using fluorescein–avidin and plotted against cholesterol content. The values are expressed as percentage of nontreated control. Cholesterol in untreated platelets was 33 mol% of total lipids. (b) BCθ bound to cells was retained in the Tx-insoluble fraction. After incubation with BCθ, platelets, erythrocytes, MOLT-4, and A431 cells were extracted with 1% Tx (4°C or 37°C) or 60 mM octyl glucoside (OGD) (4°C) and the insoluble fraction was separated by centrifugation at 15,000 × g. The distribution of BCθ in insoluble pellet and soluble fractions were determined. T, total; P, pellet; S, soluble fractions.