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. 2001 Apr 17;98(9):4926–4931. doi: 10.1073/pnas.091090798

Figure 2.

Figure 2

BCθ is enriched in FLDF. BCθ-bound platelets were treated with (+Tx) or without Tx (-Tx), sonicated, subjected to a sucrose gradient centrifugation, and fractionated from the top (fractions 1–11 and pellet, fraction 12). The distribution of BCθ (a, +Tx and -Tx), cholesterol (c, open bars, +Tx and -Tx), and total protein (c, closed circles, +Tx and -Tx) in the gradient fractions was analyzed. In parallel experiments the distribution of sphingomyelin (SM) (b, +Tx and -Tx) and tyrosine-kinases (e, +Tx) was determined. (d) The Tx-insoluble pellet obtained at 4°C by centrifugation at 15,000 × g was subjected to density gradient fractionation and the cholesterol content in each fraction was determined. T, total platelets. On BCθ binding a shift in the distribution patterns of lipids and proteins was observed from fractions 2–4 to fractions 3–5 (+Tx). Because Tx in bottom fractions (7–12) interferes with the separation of lipids on TLC plates, the distribution pattern of sphingomyelin in these fractions was not shown (b, +Tx).