Skip to main content
. 2001 Apr 17;98(9):4926–4931. doi: 10.1073/pnas.091090798

Figure 4.

Figure 4

Cholesterol depletion with 2OHpβCD completely reduces BCθ binding to FLDF. Platelets were incubated with 20 mM 2OHpβCD or buffer alone, followed by incubation with BCθ. Cell homogenates were fractionated into a sucrose gradient and analyzed as in Fig. 2. (a) BCθ binding to cholesterol-depleted (20 mM) and control (0 mM) platelets (Tx-treated). (b) Sphingomyelin distribution in cholesterol-depleted platelets treated with (+Tx) or without Tx (-Tx). (c) Cholesterol distribution in 2OHpβCD-treated (closed circles) or untreated (open circles) samples (+Tx). (d) The distribution of tyrosine-kinases in FLDF and bottom fractions (+Tx) of 2OHpβCD-treated (20mM CD) and untreated (0mM CD) platelets. T, total platelets. Distribution of sphingomyelin and membrane proteins was determined in platelets that were not incubated with BCθ as detailed in Fig. 2's legend.