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(A) Gelatin gel zymography revealed increased levels of MMP-9 activity in the heart, liver, and lung (quantifications to the right) after hemorrhagic shock. There was no change in MMP-2. (B) Immunoblot detection of MMP-9 was upregulated in brain (bands detected with femto ECL substrate), heart, liver, and lung (bands detectable by pico ECL substrate). N = 5 rats per group, ≠p<0.05 by t-test vs. No-HS.
