Figure 6. MADM-Tet combines MADM with a binary expression system.

A) Schematic representation of MADM-Tet starting with the following genotype: R26^TG/G-tTA2;Nestin-Cre^+/−;TRE-KZ^+/−. Although all cells contain the Nestin-Cre and TRE-KZ transgenes, for simplicity they are displayed within the cells only when they are active. B) Confocal images of tissue sections stained with antibodies against GFP (green) and lacZ (red) from mice of the genotype indicated above. Note that the two markers exhibit different subcellular distribution: GFP labels whole cells including nuclei, whereas tau-lacZ is absent from the nuclei and labels the processes more strongly (an example of a red-only cell body is indicated by an arrowhead). Scale bars, left and middle panels: 50 µm, right panel: 25 µm.