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. 2012 Mar 27;7(3):e33890. doi: 10.1371/journal.pone.0033890

Figure 3. Genomic DNA gel blot analysis and organ-specific expression of BnAP2 in Brassica napus.

Figure 3

A, DNA gel blot analysis of the BnAP2 gene. Genomic DNA (30 ug) was digested with BamHI (B), EcoRI (E), and HindIII (H), and gel separated. The DNA gel blots were hybridized with the full length BnAP2 cDNA. B, RT-PCR of RNA isolated from B. napus (Zhongshuang 6) petal (P), sepal (SE), stamen (ST), and pistil (PI) using gene-specific primers for BnAP2. The loading control was Actin. C, Western blot analysis of the expression of BnAP2 protein in flower buds (B), stamen (ST), pistil (PI) in Brassica napus with the antibody against AtAP2 proein. D, RT-PCR of RNA isolated from B. napus (Zhongshuang 6) root (R), stem (ST), leaf (L), bud (B) and silique (S) using gene-specific primers for BnAP2. The loading control was Actin.