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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Differentiation. 2012 Feb 28;83(4):169–178. doi: 10.1016/j.diff.2011.12.005

Fig. 2. Tobacco smoke exposure does not affect apoptosis in differentiating human embryonic stem cells.

Fig. 2

hESCs were cultured in differentiation conditions for 8, 14, or 21 days in control, nicotine-supplemented, or tobacco smoke-exposed media, and apoptosis was quantified by flow cytometric analysis of TUNEL staining. Data shown are mean±SD (N=3). Apoptosis levels were similar in all conditions at each time point, and an increase in apoptosis was observed with progress of differentiation.