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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Acta Biomater. 2012 Feb 2;8(5):1939–1947. doi: 10.1016/j.actbio.2012.01.033

Fig. 8.

Fig. 8

Apoptosis and cell viability as estimated by flow cytometry after culturing. 200,000 cells per well with 50 μg ml−1 nanodiamonds in MEM containing 10% FCS were cultured for 24 h on a 24-well plate. After washing with 250 μl PBS, cells were labeled with propidium iodide and Annexin V-FITC (1:100). Among the apoptotic dye-positive cells, Annexin V-FITC labeled cells were plotted as early apoptotic cells and propidium iodide + Annexin-V-FITC labeled cells were plotted as late apoptotic cells. Unlabeled (non-apoptotic) cells were plotted for cell viability. *P < 0.05 vs. serum-only or PBS control.