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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Neuropharmacology. 2012 Feb 22;62(7):2429–2438. doi: 10.1016/j.neuropharm.2012.02.017

Figure 6. Dynamic treatment dependent regulation of isoform specific PKC activity and protein expression.

Figure 6

A) Main effect for treatment dependent decrease in PKCα protein expression (n = 4 in all conditions; ~82 kD, 10μg protein/lane). B) Treatment dependent decreases of PKCγ protein expression during both CIE and WD (n = 4 in all conditions, * = p < 0.05, # = p < 0.01; ~78 kD, 10 μg protein/lane). C) Increased Ng Ser36 phosphorylation during CIE (CON, n = 8; CIE, n = 9; WD, n = 7; * = p < 0.05; ~7 kD, 10 μg protein/lane), with no change in total Ng protein (~7 kD; 10 μg protein/lane). D) (CON, n = 8; CIE, n = 9; WD, n = 9). Ng Ser36 phosphorylation is PKC dependent, and indicates that despite decreased PKC protein expression during CIE/WD, the activity of PKC is increased during CIE. Both PKC protein and activity are down regulated during WD.