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. Author manuscript; available in PMC: 2013 Mar 27.
Published in final edited form as: Biochemistry. 2012 Mar 15;51(12):2551–2557. doi: 10.1021/bi300200p

Figure 3.

Figure 3

Barrier protective activity of FXa derivatives in endothelial cells in response to thrombin. Confluent EA. hy926 cells in a dual chamber system were preincubated for 3h with FXa derivatives (25 nM), followed by incubation with 5 nM thrombin for 15 min to induce hyperpermeability. Cell permeability was assessed by the amount of BSA-bound Evans blue diffused into the lower chamber by measuring OD650 nm as described under “Materials and Methods”. Each point represents Mean ± SD of three independent wells. Unpaired t-test suggests statistical significance with *; P < 0.05 vs. no FXa.