Skip to main content
. Author manuscript; available in PMC: 2013 Mar 30.
Published in final edited form as: Immunol Lett. 2012 Feb 24;143(1):122–130. doi: 10.1016/j.imlet.2012.02.009

Figure 3. Effects of SHIP and Dok gene ablation on CD4 mediated inhibition of activation through the TCR.

Figure 3

Purified CD4+ T cells from SHIP +/+ or SHIP−/− mice (A) and Dok+/+ or Dok−/− mice (B) were stimulated through CD4 as described. In three separate experiments SHIP, SHIP-2, Dok, and Dok-2 were immunoprecipitated, and precipitates were analyzed using phosphotyrosine-, SHIP-1, SHIP-2, Dok-1, or Dok-2-specific antibodies, with similar results. Ratios (phosphorylation/protein levels) were determined using NIH Image software.