Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Mar 21;8(4):498–511. doi: 10.7150/ijbs.3723

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

PMC Copyright notice

In vitro differentiation analyses for the three germ layers in the 12^th passage bovine iPS cells. scale bar: 100 μm. (A1) EBs expressing marker α-Actinin (Sarcomeric, mesoderm) are shown. (A2) The immunofluorescence staining of mesoderm marker α-Actinin is shown. (A3) EGFP expression in EBs is shown. (A4)The merge is shown. (B1) EBs expressing marker Neurofilament (ectoderm) are shown. (B2)The immunofluorescence staining of ectoderm marker Neurofilament is shown. (B3) EGFP expression in EBs is shown. (B4)The merge is shown. (C1) EBs expressing marker a-Fetoprotein(AFP, endoderm) are shown. (C2) The immunofluorescence staining of endoderm marker a-Fetoprotein is shown. (C3) EGFP expression in EBs is shown. (C4)The merge is shown.

In vitro differentiation analyses for the three germ layers in the 12^th passage bovine iPS cells. scale bar: 100 μm. (A1) EBs expressing marker α-Actinin (Sarcomeric, mesoderm) are shown. (A2) The immunofluorescence staining of mesoderm marker α-Actinin is shown. (A3) EGFP expression in EBs is shown. (A4)The merge is shown. (B1) EBs expressing marker Neurofilament (ectoderm) are shown. (B2)The immunofluorescence staining of ectoderm marker Neurofilament is shown. (B3) EGFP expression in EBs is shown. (B4)The merge is shown. (C1) EBs expressing marker a-Fetoprotein(AFP, endoderm) are shown. (C2) The immunofluorescence staining of endoderm marker a-Fetoprotein is shown. (C3) EGFP expression in EBs is shown. (C4)The merge is shown.