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. 2001 Apr 10;98(9):4938–4943. doi: 10.1073/pnas.081079298

Figure 4.

Figure 4

Yeast Hsp70 binds to ScOrc4p and RepA in vitro. (a) SDS/PAGE of proteins from yeast WCE bound to Ni2+-Sepharose saturated with His6-tagged ScOrc4p or RepA (major bands). (Top) Western blot with anti-Hsp70 mAb. (b) Immunoprecipitation with anti-Hsp70 of mixtures of WCE and proteins shown in a. (Center) SDS/PAGE. Unfilled arrows point to IgG heavy (H) and light (L) chains; full-length ScOrc4p, ΔN366, and RepA are hidden under them (see next). (Bottom) Western blot with horseradish peroxidase–anti-His mAb. (Top) Membrane was reprobed with anti-Hsp70 mAb.