Single-particle reconstruction results from the EMDB (emdatabank.org; accessed 11/30/2011), classified by the detector type used for data collection. The three published DDD reconstructions are specifically labeled: ε15 (Fig. 5), P22 (Fig. 6), and GroEL (Milazzo et al., 2011). Note that the method for estimating resolution varies, including 0.5 FSC (square), 0.143 FSC (diamond), and other methods (circle). The solid curves show approximate resolution limits for each detector type: CCD (15 µm pixel size limited to 2/3 Nyquist frequency), film (6.35 µm limited to 2/3 Nyquist frequency), and DDD (6 µm pixel size limited to 4/5 Nyquist frequency).