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. Author manuscript; available in PMC: 2012 Mar 28.
Published in final edited form as: Cell. 2005 Apr 8;121(1):101–113. doi: 10.1016/j.cell.2005.01.035

Figure 4. Normal Smad-Dependent TGFβ and BMP Response in Smurf1−/− Osteoblasts.

Figure 4

(A) Smad-dependent TGFβ or BMP signaling in osteoblasts measured by (CAGA)12-Luc or BRE-luc transcription reporter assay.

(B) Western analyses of TGFβ-induced Smad2 phosphorylation and the steady-state levels of the endogenous Smad2 and Smad3.

(C) Western analyses of BMP-2-induced Smad1/5 phosphorylation and the steady-state levels of the endogenous total Smad1/5, BMP receptors (BMPRIA and BMPRIB), and Runx2.

(D) Effect of loss of Smurf1 on transcription from the Runx2-dependent osteocalcin promoter (OC-Luc).

(E) Effect of loss of Smurf1 on transcription from 3TP-Lux. Open bar, wild-type; closed bar, Smurf1−/−.