Table 1.

Interaction of Ras(wt) and Ras(T35S) with different effectors

H-Ras-variant	Effector	KD/μM	Relative affinity K (mutant/wt)
Ras(wt)⋅mGppNHp	Raf-RBD	0.02
	RalGDS-RBD	1.0
	AF6-RBD	3.0
	Byr2-RBD	0.3
Ras(T35S)⋅mGppNHp	Raf-RBD	1.2	60
	RalGDS-RBD	>100	>29
	AF6-RBD	>100	>25
	Byr2-RBD	3.7	12

The apparent dissociation constants K_D were determined by the GDI method at 37°C, according to Herrmann et al. (17).