Table 2.

³¹P chemical shifts and conformational states of Ras and Ras mutants in the presence and absence of different RBDs

	α phosphate		β phosphate		γ phosphate	K*
	δ1/ppm	δ2/ppm	δ1/ppm	δ2/ppm	δ1,2/ppm
Ras(wt)⋅GppNHp	−11.20	−11.70	−2.56	−3.31	−0.25	1.09
+ Raf-RBD*		−11.7		−3.6	−0.3
+ RalGDS-RBD†		−11.6		−3.4	−0.4
Ras(T35S)⋅GppNHp	−11.10		−2.57		−0.27	<0.05
+ Raf-RBD		−11.60		−3.41	−0.29
+ RalGDS-RBD		−11.53		−3.26	−0.25
Ras(T35A)⋅GppNHp	−11.09		−2.49		−0.33	<0.05
+ Raf-RBD	−10.52		−2.69		−0.35
+ RalGDS-RBD	−11.09		−2.49		−0.32

Data were recorded at 5°C and pH 7.4. The equilibrium constant K* between states 1 and 2 is calculated from integrals of the β resonances defined by K* = k₁/k₋₁ = [(2)]/[(1)]. 

^*Data from Geyer et al. (7). 

^† Data from Geyer et al. (38).