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. 2012 Mar 28;2:341. doi: 10.1038/srep00341

Figure 4. Binding of mAbs to live N. meningitidis strains as measured by flow cytometry.

Figure 4

Panel a, Comparison of JAR 41 binding (25 µg/ml) to wild-type strain H44/76 (H44/76-WT, solid black line) with fHbp ID 1 in variant group 1, or an isogenic mutant with lower expression of fHbp ID 1 (H44/76-LE, blue dashed line). Solid green line, H44/76-WT incubated with 25 µg/ml of a negative control IgG mAb (JAR 11, specific for fHbp in variant groups 2 and 3); green-shaded area, H44/76-LE incubated with JAR 11. Panel b, binding of a control anti-PorA mAb. Orange line, H44/76-WT; orange-shaded area, H44/76-LE. Panels c-f, Concentration-dependent binding of JAR 41 with strain H44/76-WT (panel c), mutant strain H44/76-LE (panel d), strain 8047 with fHbp in variant group 2 (panel e), or M1239 with fHbp in variant group 3 (panel f). Panels c, d and e, thick black line, 25 µg/ml of JAR 41; dark grey-shaded area, 5 µg/ml; dashed black line, 1 µg/ml; thin black line, 0.2 µg/ml; light grey-shaded area, 25 µg/ml JAR 41 with a negative control H44/76 fHbp knock-out mutant; solid blue line, 25 µg/ml of JAR 5, which is specific for fHbp in variant group 1 and a negative control mAb for variants 2 and 3. Panel f, thick black line, 50 µg/ml of JAR 41 with wild-type strain M1239 with fHbp in variant group 3; dark shaded area, 5 µg/ml of JAR 41 (lower concentrations of JAR 41 were not tested); blue line, 50 µg/ml of JAR 5 (specific for fHbp variant group 1); Light gray shade area, 50 µg/ml of JAR 41 incubated with M1239 fHbp knock-out mutant.