Table 1. Recombinant fHbp amino acid sequence variants tested for JAR 41 binding.
| Control Anti-fHbp mAb Reactivity5 | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Source Strain (fHbp gene) | fHbp ID1 | Sub-family2 | Variant Group3 | Modular Group4 | JAR 1 | JAR 4 | JAR 3/56 | mAb 502 | JAR 11 | JAR 13 | JAR 31 | JAR 35 |
| MC58 | 1 | B | 1 | I | + | + | + | + | − | − | − | − |
| Synthesized7 | 2 | B | 1 | 1 | + | + | + | − | − | − | − | − |
| SK068 | 4 | B | 1 | I | + | + | + | + | − | − | − | − |
| M6190 | 6 | B | 1 | I | − | + | − | − | − | − | − | − |
| Mali 29/07 | 9 | B | 1 | I | + | + | + | + | − | − | − | − |
| SK105 | 13 | B | 1 | I | + | + | + | + | − | − | − | − |
| NZ98/254 | 14 | B | 1 | I | − | + | + | − | − | − | − | − |
| Uganda 6/07 | 74 | B | 1 | I | − | + | + | − | − | − | − | − |
| NM452 | 15 | B | 1 | IV | − | − | + | − | − | − | − | − |
| CDC-1573 | 55 | B | 1 | IV | − | − | − | − | − | − | − | − |
| RM1090 | 22 | A | 2 | III | − | + | − | − | − | + | + | + |
| SK139 | 19 | A | 2 | VI | − | + | − | − | + | + | + | − |
| 8047 | 77 | A | 2 | VI | − | + | − | − | + | + | + | − |
| M1239 | 28 | A | 3 | II | − | − | − | − | − | + | + | + |
| 2040 | 111 | A | 3 | II | − | − | − | − | − | + | + | − |
| N27/00 | 45 | A | 3 | V | − | − | − | − | + | + | + | − |
| S3032 | 79 | A | 3 | V | − | − | − | − | + | + | + | − |
| MA-5756 | 67 | A | 3 | VIII | − | − | − | − | − | + | + | + |
| 19498 | 175 | A | 3 | IX | − | − | − | − | + | + | + | − |
| 0167/03 | 207 | A/B | 1/2 | VII | − | + | + | − | − | − | + | − |
| Synthesized7 | 283 | A/B | 1/3 | X | − | − | − | − | − | − | − | − |
1Amino acid sequence variant in the public database, http://pubmlst.org/neisseria/fHbp/.
2Sub-family as described by Murphy et al11.
3Variant group as described by Masignani et al12.
5Positive reactivity, OD >1 in an ELISA when tested at 0.1 µg/ml; negative reactivity, OD<0.3 when tested at 1 µg/ml. JAR 1, JAR 4, JAR 3 and JAR 515,16, and mAb50231,37 are from mice immunized with fHbp ID 1 (variant group 1). JAR 11 and JAR 13 are from a mouse immunized with fHbp ID 16 in variant group 216,42, and JAR 35 (REF:Beernink 2008) and JAR 31 was from a mouse immunized with fHbp ID 28 (variant group 3) (unpublished data of D.M.G.).
6JAR 3 and JAR 5 recognize overlapping epitopes involving Arg at position 121 of fHbp ID 116.
7Genes encoding fHbp IDs 2 and 283 were synthesized from nucleic acid sequences of fHbp alleles 2 and 333, respectively, obtained from public database, http://pubmlst.org/neisseria/fHbp/.