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. 2012 Mar 14;61(4):866–876. doi: 10.2337/db10-1492

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© 2012 by the American Diabetes Association.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

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FIG. 2. — Rapid revascularization of the graft by EPC cotransplantation. A and B: The blood vessels of the graft site were visualized at day 35 using BS1-lectin and VE-cadherin immunostaining (n = 4). A: Representative images of the immunostaining. The areas in the white dotted rectangle are shown as magnification view in the right panel. B: The total area of all vessels, large-vessel (diameter ≥5 μm) and small-capillary (diameter <5 μm), in the graft were quantified. C–F: The BS1-lectin–positive blood vessels of the graft were shown from early to late time point (days 3, 14, 21, and 35). C: Representative images of the immunostaining. D–F: The area of total BS1-lectin–positive vessels (D), branching vessels longer than 30 μm (E), and capillary density (F) were measured in the graft site (n = 4). All quantification data of vascular density in B, D, E, and F were calculated as percent area of the total graft area and then presented as arbitrary units (AU), with the value of the islet-only group set to 1 in B, with the value of the islet-only group at day 3 set to 1 in D and E, and with the value of the islet-only group at day 14 set to 1 in F. G and H: The capillary density of the intraislet area at day 21 was visualized with anti-human and anti-pig/mouse PECAM-1 antibodies (red) together with insulin antibody (green). G: Representative images of the PECAM-1 immunostaining. H: The capillary density positive for PECAM-1 within the insulin-positive area was measured, calculated as percent area of the insulin-positive area, and then presented as arbitrary units with the value of the islet-only group set to 1 (n = 4). I: The graft sites of each mouse at day 10 were dissected, harvested, and analyzed for PECAM-1 with primers all for human, porcine, and mouse by q-PCR (n = 4). All of the q-PCR data are presented in arbitrary units after normalization to glyceraldehyde-3-phosphate dehydrogenase, with the islet-only group set to 1. The data are presented as means ± SE. *P < 0.05 vs. the islet-only group. White dotted lines show the territory of the graft site. Scale bars, 50 μm in A and 100 μm in C and G. (A high-quality color representation of this figure is available in the online issue.)