FIG. 4.
A: TaqMan gene expression of protein involved in calcium handling and contractility. eNOS was significantly upregulated by metformin, while R-SHHF rats displayed a significant increase of the PLB-to-SERCA2 ratio. As expected, Western blot analysis of phosphorylated (p) and total AMPK ratio (B) showed a marked increase in the ratio of pAMPK to total AMPK in M-SHHF rats. A representative blot is depicted. MHC, major histocompatibility complex; ANP, atrial natriuretic peptide; NCX1, Na-Ca exchanger 1; C, Sprague-Dawley rats. *P < 0.05 vs. SHHF.