Skip to main content
. 2012 Mar 14;61(4):838–847. doi: 10.2337/db11-1098

FIG. 1.

FIG. 1.

HS+MES treatment induces HSP72 and suppresses p-JNK in MIN6 cells. A: MIN6 cells were treated with HS and/or MES for 10 min. At 10 h after the treatment, the cells were harvested, and the HSP72 protein levels were determined by Western blotting. B: After HS+MES treatment, MIN6 cells were incubated with or without TNF-α (25 ng/mL) for 6 h. The expression levels of HSP72, JNK, p-JNK, BiP, and cleaved caspase-3 were determined by Western blotting. Upon insulin stimulation, p-Akt levels were also investigated. C: HSP72 protein was overexpressed by transfection of an Hsp72 cDNA-containing plasmid. TNF-α stimulation was carried out and the expression levels of HSP72, JNK, p-JNK, BiP, and cleaved caspase-3 were determined as described for B. D: Upon HS+MES with TNF-α treatment, s-siRNA or Hsp72 si-RNA was transfected. HSP72, JNK, p-JNK, BiP, and cleaved caspase-3 were determined by Western blotting. E: Quantitative results of p-JNK levels in the data shown in B. F: Quantitative results of p-JNK levels in the data shown in D. **P < 0.01; NS vs. indicated group.