Figure 4.

Comparison of chemical shifts of apo- and holo-ACP . a) An overlay of [¹⁵N, ¹ IH] TROSY spectra of uniformly ¹⁵N-labeled apo ACP_I (black) and ¹⁵N-labeled holo ACP_I (red). The assignments of the amino acids undergoing the strongest chemical shift perturbations are indicated. b) Plot of chemical shift differences between holo ACP_I and apo ACP_I. The secondary structure elements are indicated below the sequence. No CSP is given for Ile1990, which could not be assigned in the apo form.

140×71mm (300 × 300 DPI)