Figure 6.

Isotope filtered NOESY experiments for the structure determination of HMG-ACP_I a) F1-F3 strips from a 3D F1-¹³C/¹⁵N filtered, F3-¹³C-separated NOESY-HSQC of holo and HMG-ACP_I filtering the ¹³CH-¹⁵NH NOEs. The protein was loaded in vitro and the substrate is therefore unlabeled giving strong NOEs from ¹²CH to the ¹³CH groups of the protein surface. b) The cofactor S-HMG and its numeration; c) Expansion of the two-dimensional F2-filtered NOESY spectrum of ¹³C,¹⁵N-labeled HMG-ACP_I. The 4′-Ppant chain and the HMG group are unlabeled and give rise to strong NOE connectivities within the 4′-Ppant arm.

140×100mm (300 × 300 DPI)