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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Ann Neurol. 2012 Jan 23;71(3):314–322. doi: 10.1002/ana.22657

Figure 5. Blocking antibody reduces NMO-like lesions produced by NMO-IgG and human complement in ex vivo spinal cord slice cultures.

Figure 5

a. Ex vivo spinal cord slice culture model in which slices were cultured for 7 days, followed by 3 days in the presence of NMO-IgG (purified IgG from NMO serum) and human complement, without or with blocking antibody (rAbmut). Immunostaining shown for AQP4, GFAP and myelin. Controls include non-NMO IgG, NMO-IgG or rAbmut alone, rAbmut with complement, and slice cultures from AQP4 null mice. b. NMO lesion scores (see Methods) (S.E.M., n=4−5, * P < 0.001).